Effect of Rinse Solutions on Rhizostoma pulmo (Cnidaria: Scyphozoa) Stings and the Ineffective Role of Vinegar in Scyphozoan Jellyfish Species.

Rhizostoma pulmo is a widely distributed scyphozoan in the Mediterranean Sea. Their stings result mainly in erythema, small vesicles, or/and pain, and cause a high number of bathers to seek assistance from first-aid services during the summer season. Despite the threat that jellyfish stings represent to public health, there is disagreement in the scientific community on first-aid protocols, with the dispute largely centered around the effectiveness of vinegar. In the present research, we investigated the effect of commonly used rinse solutions on nematocyst discharge in R. pulmo and the effect of vinegar on three more scyphozoans (Aurelia sp., Cassiopea sp., and Rhizostoma luteum). Scented ammonia, vinegar, and acetic acid triggered nematocyst discharge in R. pulmo. Vinegar also caused nematocyst discharge in Aurelia sp., Cassiopea sp., and R. luteum. In contrast, seawater, baking soda, freshwater, urine, and hydrogen peroxide were considered neutral solutions that did not induce nematocyst discharge. These results indicate that the use of vinegar, acetic acid, or commercial products based on these compounds is counterproductive. Their use can worsen pain and discomfort caused not only by R. pulmo stings but also by those of any scyphozoan. The use of seawater is recommended for cleaning the R. pulmo sting site until an inhibitor solution that irreversibly prevents nematocyst discharge is discovered.

The role of oceanographic conditions and colony size in shaping the spatial structure of Pyrosoma atlanticum in the NW Mediterranean Sea.

This study investigates the role of winter oceanographic conditions on the horizontal and vertical spatial structure of Pyrosoma atlanticum at different ontogenetic stages. Data were obtained on two oceanographic cruises (February 2017 and 2018) in the NW Mediterranean. Small colonies were exceptionally abundant in 2017, linked to an earlier development of spring conditions and the subsequent seasonal phytoplankton bloom. The mesoscale distribution of P. atlanticum differed depending on the colony size. Large colonies (≥7 mm) were found on the slope all along the density front, whereas small (<4 mm) and medium colonies (4-6.9 mm) extended their distribution over the shelf because of instabilities of the front, and were mostly absent in the cold, low-salinity coastal waters. The analysis of their vertical distribution showed that at night colonies of all sizes remained close to the surface, where chlorophyll-a levels were high, whereas during the day they migrated to deeper layers, reaching greater depths as the colony size increased. The migratory behaviour started when colonies were 4-6.9 mm long. The relative importance of the species in the downward carbon transport is discussed. Our results highlight the need to further study the ecology of this efficient filter feeder in the Mediterranean.

Inhibition of Nematocyst Discharge from Pelagia noctiluca (Cnidaria: Scyphozoa)-Prevention Measures against Jellyfish Stings.

Pelagia noctiluca stings are common in Mediterranean coastal areas and, although the venom is non-lethal, they are painful. Due to its high toxicity and abundance, P. noctiluca is considered a target species for the focus of research on active ingredients to reduce the symptoms of its sting. To determine the effect of 31 substances and formulations on nematocyst discharge, we performed three tests: (1) screening of per se discharge activator solutions, (2) inhibitory test with nematocyst chemical stimulation (5% acetic acid) and (3) inhibitory test quantifying the hemolytic area. Ammonia, barium chloride, bleach, scented ammonia, carbonated cola, lemon juice, sodium chloride and papain triggered nematocyst discharge. All of them were ruled out as potential inhibitors. Butylene glycol showed a reduction in nematocyst discharge, while the formulations of 10% lidocaine in ethanol, 1.5% hydroxyacetophenone in distilled water + butylene glycol, and 3% Symsitive® in butylene glycol inhibited nematocyst discharge. These last results were subsequently correlated with a significant decrease in hemolytic area in the venom assays versus seawater, a neutral solution. The presented data represent a first step in research to develop preventive products for jellyfish stings while at the same time attempting to clarify some uncertainties about the role of various topical solutions in P. noctiluca first-aid protocols.

Unravelling the ecological impacts of large-scale offshore wind farms in the Mediterranean Sea.

The need for alternative energy systems like offshore wind power to move towards the Green Deal objectives is undeniable. However, it is also increasingly clear that biodiversity loss and climate change are interconnected issues that must be tackled in unison. In this paper we highlight that offshore wind farms (OWF) in the Mediterranean Sea (MS) pose serious environmental risks to the seabed and the biodiversity of many areas due to the particular ecological and socioeconomic characteristics and vulnerability of this semi-enclosed sea. The MS hosts a high diversity of species and habitats, many of which are threatened. Furthermore, valuable species, habitats, and seascapes for citizens' health and well-being coexist with compounding effects of other economic activities (cruises, maritime transport, tourism activities, fisheries and aquaculture) in a busy space on a narrower continental shelf than in other European seas. We argue that simply importing the OWF models from the northern European seas, which are mostly based on large scale projects, to other seas like the Mediterranean is not straightforward. The risks of implementing these wind farms in the MS have not yet been well evaluated and, considering the Precautionary Principle incorporated into the Marine Strategy Framework Directive and the Maritime Spatial Planning Directive, they should not be ignored. We propose that OWF development in the MS should be excluded from high biodiversity areas containing sensitive and threatened species and habitats, particularly those situated inside or in the vicinity of Marine Protected Areas or areas with valuable seascapes. In the absence of a clearer and comprehensive EU planning of wind farms in the MS, the trade-off between the benefits (climate goals) and risks (environmental and socioeconomic impacts) of OWF could be unbalanced in favor of the risks.

Trial Assay for Safe First-Aid Protocol for the Stinging Sea Anemone Anemonia viridis (Cnidaria: Anthozoa) and a Severe Toxic Reaction.

Anemonia viridis is an abundant and widely distributed temperate sea anemone that can form dense congregations of individuals. Despite the potential severity of its sting, few detailed cases have been reported. We report a case of a severe toxic reaction following an A. viridis sting in a 35-year-old oceanographer. She developed severe pain, itching, redness, and burning sensation, which worsened one week after treatment with anti-inflammatories, antihistamines and corticosteroids. Prompted by this event, and due to the insufficient risk prevention, lack of training for marine-environment users, and lack of research into sting-specific first-aid protocols, we evaluated the cnidocyst response to five different compounds commonly recommended as rinse solutions in first-aid protocols (seawater, vinegar, ammonia, baking soda, and freshwater) by means of the Tentacle Solution Assay. Vinegar and ammonia triggered an immediate and massive cnidocyst discharge after their application and were classified as activator solutions. Baking soda and freshwater were also classified as activator solutions, although with a lower intensity of discharge. Only seawater was classified as a neutral solution and therefore recommended as a rinse solution after A. viridis sting, at least until an inhibitory solution is discovered.

Differing Effects of Vinegar on Pelagia noctiluca (Cnidaria: Scyphozoa) and Carybdea marsupialis (Cnidaria: Cubozoa) Stings-Implications for First Aid Protocols.

The jellyfish species that inhabit the Mediterranean coastal waters are not lethal, but their stings can cause severe pain and systemic effects that pose a health risk to humans. Despite the frequent occurrence of jellyfish stings, currently no consensus exists among the scientific community regarding the most appropriate first-aid protocol. Over the years, several different rinse solutions have been proposed. Vinegar, or acetic acid, is one of the most established of these solutions, with efficacy data published. We investigated the effect of vinegar and seawater on the nematocyst discharge process in two species representative of the Mediterranean region: Pelagia noctiluca (Scyphozoa) and Carybdea marsupialis (Cubozoa), by means of (1) direct observation of nematocyst discharge on light microscopy (tentacle solution assay) and (2) quantification of hemolytic area (tentacle skin blood agarose assay). In both species, nematocyst discharge was not stimulated by seawater, which was classified as a neutral solution. In P. noctiluca, vinegar produced nematocyst discharge per se, but inhibited nematocyst discharge from C. marsupialis. These results suggest that the use of vinegar cannot be universally recommended. Whereas in case of a cubozoan C. marsupialis sting, the inhibitory effect of vinegar makes it the ideal rinse solution, in case of a scyphozoan P. noctiluca sting, vinegar application may be counterproductive, worsening the pain and discomfort of the stung area.

Spatial heterogeneity of Pelagia noctiluca ephyrae linked to water masses in the Western Mediterranean.

Pelagia noctiluca is the most common jellyfish in the Western Mediterranean Sea, living in oceanic waters with a holoplanktonic lifecycle. Frequent outbreaks have been well documented in coastal areas, yet little is known about their offshore distribution. In this study we address the relationship between oceanographic structures and the distribution of P. noctiluca ephyrae along the central continental slope of the Western Mediterranean, covering a wide latitudinal gradient, during July-August 2016. The region is characterized by a rich and complex mesoscale surface circulation driven by the inflow of Atlantic Water into the Western Mediterranean through the Strait of Gibraltar. The results revealed a high variability in the ephyrae spatial paterns related with different water masses and the resulting mesoscale hydrographic features. Their horizontal distribution showed a clear latitudinal gradient with high abundances in the south, associated with recent Atlantic Water, and low abundances or absence in the north, in coincidence with the old Atlantic Water transported by the Northern Current. Ephyrae showed diel vertical migrations of short-extent in the first 50 m, with a wide distribution above the thermocline and the Deep Chlorophyll Maximum during daytime, being more concentrated towards the surface at night. The results suggest the population connectivity of P. noctiluca between the Atlantic and the Mediterranean. In that case, the abundance variability of the species in the Mediterranean could be modulated by its entrance associated with the inflow of Atlantic Water through the Strait of Gibraltar.

Seafloor litter sorting in different domains of Cap de Creus continental shelf and submarine canyon (NW Mediterranean Sea).

We analyzed litter occurrence in 68 underwater video transects performed on the middle/outer continental shelf and submarine canyon off Cap de Creus (NW Mediterranean), an area recently declared Site of Community Importance (SCI). Low densities of urban litter were registered on the shelf (7.2 items ha-1), increasing in abundance towards the deepest part of the submarine canyon, with 188 items ha-1 below 1000 m depth. We hypothesize that the strong bottom currents that recurrently affect this area efficiently move litter objects from the shelf towards the deep. Of all litter items, approximately 50% had a fishing-related origin, mostly longlines entangled on rocks in the canyon head and discarded trawl nets in deeper areas. Over 10% of cold-water colonies observed had longlines entangled, indicating the harmful effects of such practices over benthic habitats. These results should be considered when designing mitigation measures to reduce litter pollution in Cap de Creus SCI.

Reproduction, energy storage and metabolic requirements in a mesophotic population of the gorgonian Paramuricea macrospina.

This study examined the sexual reproductive cycle, energy storage and metabolic requirements of a Mediterranean gorgonian in a mesophotic ecosystem (~70 m depth). Paramuricea macrospina resulted to be a gonochoric internal brooding species with a 1:1 population sex ratio. Oogenesis lasted ~12-14 months, whereas spermatogenesis was significantly shorter, only lasting 6 months. Fertilization occurred during late summer (August) and larval release occurred during autumn (September-October). The organic matter and total lipid content showed a slight seasonal variability. Stable isotopic composition remained constant throughout the year, reflecting a general stability in gorgonian food sources. Conversely, the free fatty acid composition varied seasonally, reflecting changes in P. macrospina energetic demands probably related to gametogenesis and larval brooding. The reproductive ecology and biochemical composition of P. macrospina significantly differ from shallow coastal gorgonian species, reflecting the higher environmental stability of deeper environments.

Long-term changes in the planktonic cnidarian community in a mesoscale area of the NW Mediterranean.

In the present work, possible long-term changes in the planktonic cnidarian community were investigated by analyzing (1) species and community spatial distribution patterns, (2) variations in abundance and (3) changes in species richness during three mesoscale surveys representative of the climatic and anthropogenic changes that have occurred during the last three decades (years: 1983, 2004 and 2011) in the NW Mediterranean. These surveys were conducted during the summer (June) along the Catalan coast. All surveys covered the same area, used the same sampling methodology, and taxonomic identification was conducted by the same team of experts. An increase in the abundance of total cnidaria was found from 1983 to 2011. The siphonophore Muggiaea atlantica and the hydromedusa Aglaura hemistoma were the most abundant species, while Muggiaea kochii presented the largest abundance increment over time. Temperature was the main environmental parameter driving significant differences in the cnidarian community composition, abundance and spatial distribution patterns among the surveys. Our results suggest that in the current climate change scenario, warm-water species abundances will be positively favored, and the community will suffer changes in their latitudinal distribution patterns. We consider it extremely important to study and monitor gelatinous zooplankton in mesoscale spatial areas to understand not only long-term changes in abundances but also changes in their spatial distributions since spatial changes are sensitive indicators of climate change.

Pristine populations of habitat-forming gorgonian species on the Antarctic continental shelf.

Declines in the abundance of long-lived and habitat-forming species on continental shelves have attracted particular attention given their importance to ecosystem structure and function of marine habitats. The study of undisturbed habitats defined as "pristine areas" is essential in creating a frame of reference for natural habitats free of human interference. Gorgonian species are one of the key structure-forming taxa in benthic communities on the Antarctic continental shelf. Current knowledge of the diversity, distribution and demography of this group is relatively limited in Antarctica. To overcome this lack of information we present original data on pristine and remote populations of gorgonians from the Weddell Sea, some of which display the largest colony sizes ever recorded in Antarctica. We assessed the distribution patterns of seven gorgonian species, a morphogroup and a family in front of the Filchner Ronne Ice Shelf (Weddell Sea) by means of quantitative analysis of video transects. Analysis of these videos showed a total of 3140 colonies of gorgonians with the highest abundance in the southern section and a significantly clumped distribution. This study contributes to the general knowledge of pristine areas of the continental shelf and identifies the eastern Weddell Sea as a hotspot for habitat-forming species.

Environmental factors influencing the spatio-temporal distribution of Carybdea marsupialis (Lineo, 1978, Cubozoa) in South-Western Mediterranean coasts.

Jellyfish blooms cause important ecological and socio-economic problems. Among jellyfish, cubozoans are infamous for their painful, sometimes deadly, stings and are a major public concern in tropical to subtropical areas; however, there is little information about the possible causes of their outbreaks. After a bloom of the cubomedusa Carybdea marsupialis (Carybdeidae) along the coast of Denia (SW Mediterranean, Spain) in 2008 with negative consequences for local tourism, the necessity to understand the ecological restrictions on medusae abundance was evident. Here we use different models (GAM and zero-inflated models) to understand the environmental and human related factors influencing the abundance and distribution of C. marsupialis along the coast of Denia. Selected variables differed among medusae size classes, showing different environmental restriction associated to the developmental stages of the species. Variables implicated with dispersion (e.g. wind and current) affected mostly small and medium size classes. Sea surface temperature, salinity and proxies of primary production (chl a, phosphates, nitrates) were related to the abundances of small and large size classes, highlighting the roles of springtime salinity changes and increased primary production that may promote and maintain high densities of this species. The increased primary (and secondary) production due to anthropogenic impact is implicated as the factor enabling high numbers of C. marsupialis to thrive. Recommendations for monitoring blooms of this species along the study area and applicable to Mediterranean Sea include focus effort in coastal waters where productivity have been enriched by anthropogenic activities.

Limited Genetic Connectivity between Gorgonian Morphotypes along a Depth Gradient.

Gorgonian species show a high morphological variability in relation to the environment in which they live. In coastal areas, parameters such as temperature, light, currents, and food availability vary significantly with depth, potentially affecting morphology of the colonies and the structure of the populations, as well as their connectivity patterns. In tropical seas, the existence of connectivity between shallow and deep populations supported the hypothesis that the deep coral reefs could potentially act as (reproductive) refugia fostering re-colonization of shallow areas after mortality events. Moreover, this hypothesis is not so clear accepted in temperate seas. Eunicella singularis is one of the most common gorgonian species in Northwestern Mediterranean Sea, playing an important role as ecosystem engineer by providing biomass and complexity to the coralligenous habitats. It has a wide bathymetric distribution ranging from about 10 m to 100 m. Two depth-related morphotypes have been identified, differing in colony morphology, sclerite size and shape, and occurrence of symbiotic algae, but not in mitochondrial DNA haplotypes. In the present study the genetic structure of E. singularis populations along a horizontal and bathymetric gradient was assessed using microsatellites and ITS1 sequences. Restricted gene flow was found at 30-40 m depth between the two Eunicella morphotypes. Conversely, no genetic structuring has been found among shallow water populations within a spatial scale of ten kilometers. The break in gene flow between shallow and deep populations contributes to explain the morphological variability observed at different depths. Moreover, the limited vertical connectivity hinted that the refugia hypothesis does not apply to E. singularis. Re-colonization of shallow water populations, occasionally affected by mass mortality events, should then be mainly fueled by larvae from other shallow water populations.

Cold-Water Corals and Anthropogenic Impacts in La Fonera Submarine Canyon Head, Northwestern Mediterranean Sea.

We assess the occurrence and extent of cold-water coral (CWC) species Madrepora oculata and Dendrophyllia cornigera, as well as gorgonian red coral Corallium rubrum, in La Fonera canyon head (Northwestern Mediterranean Sea), as well as human impacts taking place in their habitats. Occurrence is assessed based on Remotely Operated Vehicle (ROV) video imaging. Terrain classification techniques are applied to high-resolution swath bathymetric data to obtain semi-automatic interpretative maps to identify the relationship between coral distribution patterns and canyon environments. A total of 21 ROV immersions were carried out in different canyon environments at depths ranging between 79 and 401 m. Large, healthy colonies of M. oculata occur on abrupt, protected, often overhanging, rocky sections of the canyon walls, especially in Illa Negra branch. D. cornigera is sparser and evenly distributed at depth, on relatively low sloping areas, in rocky but also partially sedimented areas. C. rubrum is most frequent between 100 and 160 m on highly sloping rocky areas. The probable extent of CWC habitats is quantified by applying a maximum entropy model to predict habitat suitability: 0.36 km2 yield M. oculata occurrence probabilities over 70%. Similar predictive models have been produced for D. cornigera and C. rubrum. All ROV transects document either the presence of litter on the seafloor or pervasive trawling marks. Nets and longlines are imaged entangled on coral colonies. Coral rubble is observed at the foot of impacted colonies. Some colonies are partially covered by sediment that could be the result of the resuspension generated by bottom trawling on neighbouring fishing grounds, which has been demonstrated to be responsible of daily increases in sediment fluxes within the canyon. The characteristics of the CWC community in La Fonera canyon are indicative that it withstands high environmental stress of both natural and human origin.

Carybdea marsupialis (Cubozoa) in the Mediterranean Sea: the first case of a sting causing cutaneous and systemic manifestations.

A woman stung by the box jellyfish Carybdea marsupialis (Cnidaria, Cubozoa) at a Spanish Mediterranean beach showed systemic manifestations over several months [pain far from the inoculation point, arthralgia, paresthesia, hyperesthesia, increase in eosinophils and immunoglobulin E (IgE)] in addition to the skin condition.

Applying cathodically polarised substrata to the restoration of a high value coral.

Larval settlement of the high value red coral, Corallium rubrum, was studied on three different CaCO(3) substrata, viz. lithogenic (marble), electro-accreted calcium carbonate in the presence and in the absence of cathodic polarisation. The last two substrata consisted of stainless steel plates galvanically coupled with Zn anodes. The electrochemical characterization of the settlement device was studied in order to investigate correlations between cathodic parameters (polarisation potential, current density, calcareous deposit composition) and larval settlement. The results obtained in the natural habitat (at 35 m depth) showed that settlement was five times lower on the electro-accreted aragonite in the presence of low cathodic current densities (i≤1 µA cm(-2)) compared to both marble tiles and electro-accreted aragonite in the absence of polarisation. These last two substrata showed similar settlement values. The implications of these findings on restoration strategies for C. rubrum are discussed.